STOmics ®  Stereo seq Translucent Reagent Kit

The STOmics® Stereo-seq clearing reagent kit is a pre-experiment reagent kit used to determine the optimal clearing time for tissue sections. The Stereo-seq Chip P (clearing test chip) contains nucleotide capture probes that bind to the tissue section. These probes capture mRNA molecules in situ within the tissue, followed by cDNA synthesis using fluorescently labeled nucleotides. Researchers can then quickly determine the optimal clearing time for specific tissues through fluorescence microscopy imaging. Choosing the optimal clearing conditions facilitates better mRNA capture. The best clearing time is determined by maintaining consistent imaging conditions (including brightness and exposure) and ensuring the tissue morphology is intact, the fluorescence signal is strongest, and there is no dispersion. When cleared for 3 minutes, the tissue exhibits uneven brightness in the same cortex, indicating insufficient clearing. At 12 minutes of clearing, the details are clear, the signal is uniform, and the brightness is maximal. At 24 minutes of clearing, the signal is weaker than that at 12 minutes. Therefore, the optimal clearing time is 12 minutes.

STOmics ®  Stereo seq Transcriptome Kit

The STOmics® Stereo-seq transcriptome reagent kit is used to construct a 3' end full-transcript library from tissue sections. The Stereo-seq Chip T (spatiotemporal poly-T chip) contains capture probes with spatial coordinate information. These probes bind to the tissue section and capture mRNA molecules in situ, followed by cDNA synthesis. Researchers can obtain ultra-high resolution spatial transcriptome information of specific samples through DNBSEQ sequencing and the complementary STOmics® visualization analysis tools.

By using the Stereo-seq library construction kit, you can generate a Stereo-seq sequencing library. This efficient biochemical workflow, combined with powerful visualization analysis tools, enables ultra-high resolution spatial omics.