The Stereo-seq Chip is used for mRNA localization, featuring a surface densely packed with DNA Nano Balls (DNBs) arranged in a regular array. Sequencing the DNBs fixed on the chip provides information that maps each sequence to a coordinate position (Coordinate ID, CID). This mapping allows for the spatial localization of mRNA captured on the chip. The sequence-to-CID mapping is stored in an h5 format file, known as a Mask file. Mask files cannot be opened directly and require processing with the Stereo-seq Analysis Workflow (SAW) software package.